Sample displacement chromatography as a method for purification of proteins and peptides from complex mixtures
نویسندگان
چکیده
منابع مشابه
Ion-Exchange Sample Displacement Chromatography as a Method for Fast and Simple Isolation of Low- and High-Abundance Proteins from Complex Biological Mixtures
Martina Srajer Gajdosik1, Spomenka Kovac1, Nela Malatesti2, Egbert Müller3 and Djuro Josic2,4* Department of Chemistry, Josip Juraj Strossmayer University, Cara Hadrijana 8/A, HR-31000 Osijek, Croatia Department of Biotechnology, University of Rijeka, Radmile Matej~i} 2, HR-51000 Rijeka, Croatia Tosoh Bioscience GmbH, Zettachring 6, DE-70567 Stuttgart, Germany Warren Alpert Medical School, Brow...
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The antibody fraction (less than 6ml) was added to immobilized enzyme (2ml of packed gel). The antibodies were recycled for 2h and the unattached antibodies washed from the gel for 1 h. Specific antibodies were eluted by washing with citric acid (0.1 M, pH2.2). Experiments marked with an asterisk were performed simultaneously. The molecular weights of immunoglobulin G and creatine kinase were t...
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ژورنال
عنوان ژورنال: Journal of Chromatography A
سال: 2012
ISSN: 0021-9673
DOI: 10.1016/j.chroma.2012.03.046